Part:BBa_K4241791

Nitrogen Starvation Inducible promoter 2 (Microalgae)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 630
  Illegal PstI site found at 679
  Illegal PstI site found at 749
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 87
  Illegal PstI site found at 630
  Illegal PstI site found at 679
  Illegal PstI site found at 749
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 238
  Illegal XhoI site found at 663
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 630
  Illegal PstI site found at 679
  Illegal PstI site found at 749
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 630
  Illegal PstI site found at 679
  Illegal PstI site found at 749
  Illegal NgoMIV site found at 902
  Illegal AgeI site found at 832
  
• 1000
  COMPATIBLE WITH RFC[1000]

Overview

This part is CvNDI2, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Usage and Biology

CvNDI2 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression. This component is a promoter region to be added before the coding sequence of the protein of interest. The activation of the promoter must be induced via nitrogen starvation.

References

Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9